Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 4;11:239–252. doi: 10.2147/OTT.S114276

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Tsai et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

PMC Copyright notice

miR-99a-5p inhibits mTOR expression.

Notes: (A) mTOR mRNA level in cells transfected with pSM or pSM-99a was detected by QPCR. Data represent mean ± SD values of three independent experiments, each performed in triplicate. *P<0.05. (B) Protein expression level of mTOR in cells transfected with pSM, pSM-99a or pSM-28-5p was detected by Western blot. A representative blot is shown in the upper panel. The EGFP, which co-expressed with miRNA transcripts, was detected, and β-actin was used as a loading control. The relative protein levels of mTOR in each treatment were quantified as shown in the lower panel. *P<0.05.

Abbreviations: mTOR, mammalian target of rapamycin; QPCR, quantitative polymerase chain reaction; EGFP, enhanced green fluorescent protein.