. 2018 Jan 4;11:239–252. doi: 10.2147/OTT.S114276

Table S1.

Sequence of oligonucleotides used in this study

Primer name	Sequence 5′→3′	Note
Paired oligonucleotides for the cloning of miRNA expression vectors
miR-99a_Top	TGCTGAACCCGTAGATCCGATCTTGTGGTTTTGGCCACTGACTGACCACAAGATGATCTACGGGTT	Matured hsa-miR-99a-5p sequence is underlined
miR-99a_Bot	CCTGAACCCGTAGATCATCTTGTGGTCAGTCAGTGGCCAAAACCACAAGATCGGATCTACGGGTTC
miR-28-5p_Top	TGCTGAAGGAGCTCACAGTCTATTGAGGTTTTGGCCACTGACTGACCTCAATAGTGTGAGCTCCTT	Matured miR-28-5psequence is underlined
miR-28-5p_Bot	CCTGAAGGAGCTCACACTATTGAGGTCAGTCAGTGGCCAAAACCTCAATAGACTGTGAGCTCCTTC
Paired oligonucleotides for the cloning of reporter constructs
mTOR-TS_Top	CTAGCTGGGGAACAGAAGATCCATAACTTTAGAAATACGGGTTTTGACTTAACG	Seed sequences of miR-99a in mTOR 3′-UTR are boxed
mTOR-TS_Bot	TCGACGTTAAGTCAAAACCCGTATTTCTAAAGTTATGGATCTTCTGTTCCCCAG	Seed sequences of miR-99a in mTOR 3′-UTR are boxed
mTOR-MTS_Top	TCGAGTGGGGAACAGAAGATCCATAACTTTAGAAAATGCCCATTTGACTTAACGC	Mutated seed sequences of mTOR are boxed
mTOR-MTS_Bot	GGCCGCGTTAAGTCAAATGGGCATTTTCTAAAGTTATGGATCTTCTGTTCCCCAC	Mutated seed sequences of mTOR are boxed
99a-PTS_Top	CTAGCCACAAGATCGGATCTACGGGTTGATATCCACAAGATCGGATCTACGGGTTATAACACAAGATCGGATCTACGGGTTC	Three miR-99a-5p positive targeting sites are underlined
99a-PTS_Bot	TCGAGAACCCGTAGATCCGATCTTGTGTTATAACCCGTAGATCCGATCTTGTGGATATCAACCCGTAGATCCGATCTTGTGG
Primers used in QPCR detection of mTOR expression
mTOR_5QP	AGACACCCATCCAACCTGAT	NM_004958; amplicon: 85 bps
mTOR_3QP	TCATAGCAACCTCAAAGCAGTC	NM_004958; amplicon: 85 bps
EGFP_5QP	CGACGGCAACTACAAGAC
EGFP_3QP	TAGTTGTACTCCAGCTTGTGC

Abbreviations: Bot, bottom; miRNA, microRNA; mTOR, mammalian target of rapamycin; 3′-UTR, 3′ untranslated region; QPCR, quantitative polymerase chain reaction; EGFP, enhanced green fluorescent protein.