Fig.2.

Standard curve fitting model evaluation. EP values obtained from the 2B7-MW1 SMC analysis of serial dilutions of N573 Q45 HTT protein were fitted using (A) linear regression, (B) four-parameter logistic (4PL), and (C) 5PL models. Each point is the mean of 3 replicates. Bars represent standard deviation. (D) % TE and % RE were evaluated for each dilution point for the three fitting models. The 4PL and 5PL models achieved less than 30% error for points within the quantification range of the assay, as required by the EMA assay validation guideline. For the 5PL model, all assayed dilutions including the one below the LLoQ, showed error <30%.