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. 2018 Jan 8;137(2):166–183. doi: 10.1161/CIRCULATIONAHA.116.023381

Figure 5.

Figure 5.

Aggrecanase expression in the porcine model of stent injury and comparison to human vascular cells. A, The aggrecan neoepitope NITEGE and the versican neoepitope DPEAAE are generated by ADAMTS cleavage. B, Aggrecan NITEGE and versican DPEAAE neoepitopes in DES at day 1, 3, 7, and 28 post–stent implantation. n=3 per time point. C, Differences between BMS and DES at day 28. n=4 per group. D, Gene expression of ADAMTS1, -4, and -5 in porcine tissue. n=3 per time point for BMS/DES; n=6 for control coronary arteries. Gene expression values were normalized to unstented control arteries. Linear regression analysis for P value for trend. E, Basal ADAMTS expression levels in porcine coronary arteries. Cycle threshold (CT) values by qPCR are given as 40-CT (higher values indicate higher expression). n=6 per group. F, Gene expression of ADAMTS1, -4, and -5 as well as versican, aggrecan, and HAPLN1 in ECs and SMCs of human coronary arteries. CT values by qPCR are given as 40-CT (higher values indicate higher expression). HCAEC indicate human coronary artery endothelial cell; HCASMC, human coronary artery SMCs; n=triplicates of 3 independent cell passages. ***P<0.001 (t test with unequal variance). BMS indicates bare-metal stent; DES, drug-eluting stent; EC, endothelial cell; qPCR, quantitative polymerase chain reaction; and SMC, smooth muscle cell.