Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 8;137(2):166–183. doi: 10.1161/CIRCULATIONAHA.116.023381

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 The Authors.

Circulation is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited.

PMC Copyright notice

Figure 6. — Aggrecan in the human vasculature. A, Localization of aggrecan, the aggrecan NITEGE, and versican DPEAAE neoepitopes (neo), and HPLN1 in stented and control human coronary arteries with the presence of atherosclerosis (*). Arrows mark the contacts of the stent struts with coronary artery. Scale bars=1 mm. B, Colocalization of aggrecan (Alexa 633, displayed in green) and aggrecan NITEGE neoepitope (Alexa 568, displayed in red) in human stented coronary arteries by immunofluorescence. Note the presence of aggrecan fragments at the contacts of the stent struts with the artery and in the subendothelial layer (zoomed-in areas). Overview image 20×, scale bar=500 µm; Zoomed-in areas 60×, scale bar=25 µm. C, Adjusted peak area for aggrecan, versican, and decorin in the human thoracic aorta and saphenous veins as determined by targeted proteomics. n=7 per group. *P<0.05, **P<0.01 (t test with unequal variance). D, Immunoblots for the aggrecan NITEGE neoepitope and versican in the human thoracic aorta and saphenous veins. The SLRP decorin served as a loading control. n=4 per group. E, Colocalization of aggrecan (Alexa 633, displayed in green) and aggrecan NITEGE neoepitope (Alexa 568, displayed in red) in human aorta visualized by immunofluorescence. Elastin fibers in white (autofluoresence with 488-nm laser excitation captured in the green emission channel). Control sections stained with isotope IgGs. Magnification 60×, scale bars=20 µm. CA indicates coronary artery; DAPI, 4′,6-diamidino-2-phenylindole; HPLN1, hyaluronan and proteoglycan link protein 1; IgG, immunoglobulin G; and SLRP, small leucine-rich proteoglycan.