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Treatment with haemofungin causes accumulation of PPIX in A. fumigatus. A. fumigatus overnight culture grown in YAG liquid medium was treated with 2 μM haemofungin for 2 h. The mycelium was (a) spun down and directly viewed for accumulation of porphyrins and (b) lyophilized and analysed for PPIX content by HPLC (C18) column separation.
