Table 1. Primer sets.

Gene	Forward primer	Reverse primer	Tm
AMH	GCATGGCCAACTGGTACACT	GATGTGTGTGTGAGGCCTTG	60
Esr1	CAGGTGCCCTACTACCTGGA	CAGTCTCTCTCGGCCACTCT	60
Esr2	TGCAGAACCTCAAAAGAGTCC	AGCATCCCTCTTTGAACTCG	60
FGF-2	GCTGCTGGCTTCTAAGTGTGT	CCAACTGGAGTATTTCCGTGA	60
FSHβ	TGCATCCTATTCTGGTGCTG	TTTCTGGGTATTGGGTCTGG	60
FSHR	TTTACTTGCCTGGAAGCGACTAA	CCCAGGCTCCTCCACACA	57
GnRH	TCTGGTCATGTTGTCCGTGT	CTTGCTGGTGTGTGGTATGC	61
HPRT-1	TGATCAGTCAACAGGGGACA	CTGGCCGATATCCAACACTT	55
Inhibin-α	CTGCCCTCAACATCTCCTTC	CTCATGCTCCCTGGTAGAGC	60
LHβ	CTGCTATGGCTGTTGCTGAG	AACACTCGGACCATGCTAGG	60
LHR	CATTCAATGGGACGACTCTA	GCCTGCAATTTGGTGGA	60
MMP-2	ATGATGTCAGCTTCCCCATC	ACCTGCACCCTGAAACAGTG	55
MMP-9	ACTTTGGAAACGCAAATGGT	AGTCTCTCACTGGGGCAGAA	61
PCNA	AGCACTCGTATTTGAAGCACCA	TCACCAGAAGGCATCTTTACCA	62

Primer sequence for Esr1, Esr1 and Cyp19a1 (Phalen et al., 2009); AMH primer sequence provided by Dr. Ned Place; GDF-9 (Wang and Roy, 2006); FSHR (Kanimozhi et al., 2014); GnRH, LHβ and FSHβ primers (Shahed and Young, 2010).