Fig. 1. BM cDC progenitors can be identified without MHCII in Zbtb46^+/GFP mice and their development is CIITA-independent.

(A) Flow cytometry analysis of BM cDC progenitors from Ciita^+/+Zbtb46^+/GFP and Ciita^−/−Zbtb46^+/GFP mice defined as Lin⁻SiglechH⁻ CD11c⁺CD135⁺CD115⁺CD117⁻Zbtb46^gfp+ and Lin⁻SiglechH⁻CD11c⁺CD135⁺CD115⁻CD117^int Zbtb46^gfp+ for pre-CD4 and pre-CD8 DCs, respectively (n = 5). Contour plots are from a single experiment representative of 2 experiments with 2–3 mice per experiment. (B) Frequency of pre-CD4 and pre-CD8 DCs in vivo as defined in. Percent for pre-CD4 DC was based on a pre-gate defined as Lin⁻SiglechH⁻CD11c⁺CD135⁺CD115⁺cells. Percent for the pre-CD8 DC was based on a pre-gate defined as SiglechH⁻CD11c⁺CD135⁺CD115⁻cells. Data are shown as a dot plot with mean bar for two pooled experiments with 2–3 mice per experiment (n = 5). (C) Output of sorted pre-CD4 and pre-CD8 DC progenitors as defined in (A) after 5 days of Flt3L-treated cultures. Shown are contour plots from a single experiment pre-gated as B220⁻SiglecH⁻CD11c⁺Zbtb46^gfp+ representative of 2 experiments with 2–3 mice per experiment (n = 5).