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. Author manuscript; available in PMC: 2018 Jan 8.
Published in final edited form as: Cell Rep. 2015 Aug 28;12(10):1575–1583. doi: 10.1016/j.celrep.2015.08.003

Figure 4. Ultrastructural Analysis and Reconstruction of Retinal Axons Contributing to “Complex Encapsulated” Retinogeniculate Synapses in dLGN.

Figure 4

(A–D) SBFSEM images of six retinal terminals synapsing onto the same relay cell dendrite (pseudo-colored in bright green) in the “shell” region of dLGN.

(E) Key indicates the types of cellular elements pseudo-colored in (A)–(D) and (F)–(H).

(F) 3D reconstruction of all of the elements pseudo-colored in (A)–(D).

(G) 3D reconstruction of three RGC axons, an inhibitory interneuron dendrite and the relay cell dendrite in (A)–(D).

(H) 3D reconstruction of a single RGC axon and the relay cell dendrite in (A)–(D). Arrow indicates a retinal bouton that makes synaptic contact with an element other than the relay cell dendrite pseudo-colored bright green.

(I–K) SBFSEM images of 14 retinal terminals synapsing onto the same relay cell dendrite (pseudo-colored in bright yellow).

(L) Key indicates the types of cellular elements pseudo-colored in (I)–(K) and (M)–(O).

(M) 3D reconstruction of all of the elements pseudo-colored in (I)–(K).

(N) 3D reconstruction of three RGC axons and the relay cell dendrite in (I)–(K).

(O) 3D reconstruction of a single RGC axon and the relay cell dendrite in (I)–(K). Arrow indicates a retinal bouton that makes synaptic contact with an element other than the relay cell dendrite pseudo-colored bright yellow. Scale bar in (D), 1.5 μm for (A)–(D), and in (K), 1.5 μm for (I)–(K).