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. 2018 Jan 8;14(1):e1006769. doi: 10.1371/journal.ppat.1006769

Fig 10. PML-NB entrapment of vDNA does not lead to the induction of innate immunity.

Fig 10

(A) Relative Mx1, ISG15, and ISG54 mRNA levels in cell lines restrictive (HFt, RPE) or permissive (U2OS, SAOS) to HSV-1 ICP0-null mutant (ΔICP0) infection. Cells were mock treated, IFN-β (100 IU/ml) stimulated, or infected with ΔICP0 at an MOI of 1 PFU/cell for 9 h. n = 3, means (RQ) and standard deviations (RQmin/max) are shown and expressed relative to mock for each cell line (1). (B) Restrictive (HFt, RPE) or permissive (U2OS, SAOS) cell lines were infected with ΔICP0 at an MOI of 1 PFU/cell and treated with either DMSO or Ruxolitinib (Ruxo, 5μM). CRV was harvested at 48 hpi and titres determined on U2OS cells. n = 3, means and standard deviations shown and expressed as relative fold-change to DMSO titres (1) for each cell line. * P < 0.05, *** P < 0.001, ns (not significant); two-tailed t-test.