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. Author manuscript; available in PMC: 2018 Jan 8.
Published in final edited form as: Cell Rep. 2017 Jul 5;20(1):224–235. doi: 10.1016/j.celrep.2017.05.070

Figure 6. Grx1 Is a Physiological Modulator of Protein Cysteine S-Glutathionylation.

Figure 6

(A) Grx activity measured in the serum of irradiated mice. Serum samples were collected at the indicated times after 4 Gy whole-body irradiation. One unit was defined as consumption of 1 μmol of NADPH per min. Data are means ± SD of n = 3 mice.

(B) Grx activity measured in the BM extracellular space of irradiated mice (number of units per milligram BM protein). Data are means ± SD of n = 3 mice.

(C) Grx staining in BM biopsies of acute myeloid leukemia (AML) patients. Images are representative of BM before (left) and 1 month after (right) chemotherapy induction.

(D) The percentage of patients showing increased Grx1 expression after induction chemotherapy (n = 27).

(E) Glutathionylation staining of WT and Grx1 KO mouse BM in the presence or absence of GSH. Representative fluorescent images are shown. Addition of excess reduced GSH to the primary GSH antibody mix abrogated glutathionylation.

(F) Quantitative analysis of protein glutathionylation in WT and Grx1 KO mouse BM. Mean fluorescence intensities (±SD) are shown. *p ≤ how versus WT.

(G) Glutathionylation staining of the BM of irradiated WT and Grx1 KO mice (4 Gy whole-body irradiation). Representative fluorescent images are shown.

(H) Quantitative analysis of protein glutathionylation in irradiated WT and Grx1 KO mouse BM. Mean fluorescence intensities (±SD) are shown. *p ≤ 0.005 versus WT.