KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Alexa Fluor 647 goat anti-mouse IgG1	Thermo Fisher Scientific	A21240; RRID:AB_2535809
Alexa Fluor 488 donkey anti-chicken IgY	Jackson Immuno Research	703-545-155; RRID:AB_2340375
Alexa Fluor 568 goat anti-mouse IgG2a	Thermo Fisher Scientific	A21134; RRID:AB_2535773
Chicken polyclonal anti-GFP antibody	Aves Labs	GFP-1020; RRID:AB_10000240
Mouse IgG2a monoclonal anti-BK channel a subunit antibody	UC Davis/NIH NeuroMab Facility	73-022; RRID:AB_10698180
Mouse IgG1 monoclonal anti-ryanodine receptor	Thermo Fisher	MA3-925;
antibody	Scientific	RRID:AB_2254138
Bacterial and Virus Strains
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
w-Agatoxin-IVA	Peptide Institute	4256-s
Alexa Fluor 594 hydrazide	Thermo Fisher Scientific	A10438
Apamin	Peptide Institute	4257-v
Cyclopiazonic acid	Sigma-Aldrich	C1530
Fluo-5F	Thermo Fisher Scientific	F14221
Iberiotoxin	Peptide Institute	4235-s
MK-801	Sigma-Aldrich	M107
NBQX disodium salt	Alamone	N-186
Picrotoxin	Sigma-Aldrich	P1675
QX-314	Alomone labs	Q-150
Ryanodine	Alomone	R-500
SR95531	Abcam	ab120042
Strychnine	Sigma-Aldrich	S0532
TetraSpeck Microspheres	Thermo Fisher Scientific	T7279
TTA-P2	Alomone	T-155
TTX	Abcam	ab120055
Ruthenium red	Wako	189-03181
Critical Commercial Assays
Deposited Data
Experimental Models: Cell Lines
Experimental Models: Organisms/Strains
Mouse (C57BL/6J)	Jackson Labs	RRID:IMSR_JAX:0006 64
Mouse (GlyT2-Cre)	MMRRC	030730-UCD; RRID:MMRRC_03073 0-UCD
Mouse (Ai32)	Jackson Labs	012569; RRID:IMSR_JAX:0125 69
Oligonucleotides
Recombinant DNA
Software and Algorithms
Igor Pro 6	Wavemetrics	RRID:SCR_000325
Neuromatic	http://www.neuromatic.thinkrandom.com	RRID:SCR_004186
Fiji	https://fiji.sc/	RRID:SCR_002285
GraphPad Prism 5	GraphPad Software	RRID:SCR_002798
Other