Figure 8. A Doc2B mutant lacking the binding sequence for Tctex-1 (Doc2BΔTCT) supports normal secretion.
(A) Ca2+ uncaging experiment in Doc2B KO cells (n = 13) and KO cells overexpressing Doc2B WT (n = 15) or Doc2BΔTCT (n = 14). (B–F) Kinetic analysis revealed that both vesicle pool sizes and kinetics of release were not significantly different between Doc2B WT and Doc2BΔTCT. *p<0.05; ***p<0.001, Dunn’s multiple comparison test.