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. 2017 Nov 20;6:e32728. doi: 10.7554/eLife.32728

Figure 4. The C-terminal region contains motifs essential for Alx1 function.

Figure 4.

(A) Constructs generated with different C-terminal deletions and truncations. (B–G) Embryos fixed at the mid-late gastrula stage and labelled with 6a9 antibody. (B’–G’) DIC/fluorescence composite images of the same embryos. (B, B’) Uninjected control embryo. (C, C’) Positive control embryo co-injected with LvAlx1 MO and LvAlx1.WT.GFP. (D, D’) Deletion of the highly conserved OAR domain does not affect rescue of PMC specification or patterning. (E, E’ and F, F’) Embryos co-injected with LvAlx1 MO and LvAlx1 mRNA lacking C-terminal sequences (LvAlx1.ΔC.GFP and LvAlx1.ΔC.ΔOAR.GFP) contain no 6a9-positive cells, similar to embryos injected with LvAlx1 MO alone (G, G’).