Figure 3.

MicroRNA‐126 (Mir‐126) deficiency promoted the pathology of CD4⁺ T cell‐mediated autoimmune colitis. MiR‐126 knock‐down (KD) mice and wild‐type (WT) mice (8–10 weeks old, n = 6) were treated with 3% dextran sulphate sodium (DSS) water for 10 days. (a) Schematic representation of the animal study. (b) Clinical and histological scores. (c) The pathology of colitis was observed by haematoxylin and eosin (H&E) staining. Magnification: ×100 and ×400. Arrows indicate intestinal tissue loss. (d) The proportion of CD4⁺ T cells in spleen was analysed by FACS and calculated. (e) The total cell number of CD4⁺ T cells was calculated. (f) The expression levels of CD62L and CD44 in CD4⁺ T cells also were analyzed by fluorescence activated cell sorting (FACS) and calculated (g). One representative example of three independent experiments is shown. *P < 0·05. [Colour figure can be viewed at wileyonlinelibrary.com]