Figure 2.
Ibuprofen treatment reduces cytokine expression in LPS‐activated macrophages. (A) Microphotograph showing morphological alterations and flattened shape (arrows) of RAW264.7 macrophages upon activation with 10 ng·mL−1 LPS for 16 h in the presence of 800 μM ibuprofen. (B) Cell diameter of LPS‐treated RAW264.7 macrophages in the presence of ibuprofen. (C) Quantification of live and dead RAW264.7 macrophages treated with LPS in the presence of ibuprofen. (D) qPCR of pro‐inflammatory markers in RAW264.7 macrophages treated with LPS in the presence of ibuprofen. Results are average ± SEM (n = 5), *P < 0.05. Scale bars: 50 μm.