Flow cytometric analysis of eosinophils in (A) blood (n=97), (B) sputum (n=94) and (C) BAL (n=91). NS, never-smokers; FS-NAO, former smokers, no airflow obstruction; CS-NAO, current smokers, no airflow obstruction; FS-COPD, former smokers with COPD; CS-COPD, current smokers with COPD. Box and whiskers plot showing median ± interquartile range, 5th and 95th percentiles, with outliers shown as symbols. *, p<0.05; **, p<0.01; ANOVA with Holm-Sidak post-hoc testing.