Table 2.
Steroid production by fast- and slow-grow follicles collected from macaque ovarian cortical tissue not treated (no-stained) or treated with neutral red (NR-stained) during 3D encapsulated culture in vitro
Follicle growth Cohorts | Groups | Week 1 | Week 2 | Week 4 |
---|---|---|---|---|
Estradiol (pg/ml) | ||||
Fast grow follicles | No-stained | 97 ± 9a | 185 ± 58b | 5613 ± 1929cA |
NR-stained | 87* | 177 ± 96b | 269 ± 107bB | |
Slow grow follicles | No-stained | 121 ± 38a | 152 ± 22a | 356 ± 99b |
NR-stained | 87 ± 4a | 145 ± 18b | 173 ± 34b | |
Androstenedione (pg/ml) | ||||
Fast grow follicles | No-stained | 37 ± 16a | 24 ± 11a | 122 ± 5bA |
NR-stained | 8 ± 3a | 30 ± 18a | 10 ± 0aB | |
Slow grow follicles | No-stained | 33 ± 7a | 7 ± 2b | 20 ± 7ab |
NR-stained | 40 ± 9a | 9 ± 3b | 19 ± 9b |
Values represent the mean ± SEM from 4 animals per treatment, 3–5 follicles for fast-grow and 18–23 slow-grow follicles per treatment group per week. Lower case letters represent differences between weeks within a group. Upper case letters represent differences between groups within week. * SEM lacking due to n = 1 in the group