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. 2018 Jan 8;8:37. doi: 10.1038/s41598-017-18280-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Examination of the expression level of long non-coding RNA (lncRNA) CASC9.5 in non-small cell lung cancer (NSCLC) cell lines using fluorescence-based quantitative polymerase chain reaction (qRT-PCR). 16HBE cells are normal bronchial epithelial cells. (B) The knockdown (KD) of the expression of lncRNA CASC9.5 in A549 and SPC-A1 cells using RNA interference (RNAi). (C) qRT-PCR analysis revealed that lncRNA CASC9.5 was primarily located in the nucleus. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a cytoplasmic marker, while U1 RNA was used as a nuclear marker. *p < 0.05, **p < 0.01, ***p < 0.001.