. 2018 Jan 8;8:28. doi: 10.1038/s41598-017-18341-7

Table 3.

Protein family hits to described proteins.

Family	DB	Tool	Best Hit (protein)	Best hit (species)	Curated Annotation
1217	nr	blastp & hmmsearch	unknown	Veillonella sp. CAG:933	Bacterial protein
532	nr	hmmsearch	hypothetical protein	M. rupellensis	Bacterial protein
565b	nr	blastp	hypothetical protein H257_12751	A. astaci	Putative replication protein, viral or bacterial
565b	nr	hmmsearch	putative replication protein	Phytophthora parasitica virus	Putative replication protein, viral or bacterial
956b	nr	blastp & hmmsearch	hypothetical protein	C. trachomatis	Torque Teno virus ORF

Four of the 32 ORFan protein families match proteins in the NR database. The table describes for each family: (1) the database of the hit, (2) the tool used to detect the similarity, (3) the description of the highest scoring hit, (4) the annotated species for the highest scoring hit, and (5) our manually curated annotation for the protein based on all the significant hits for the protein family. Manual annotation was required since the best hit for a sequence does not always correspond to the most plausible annotation, due to wrong metadata or to the discovery of a distant relative of a protein conserved in many different organisms.