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. 2018 Jan 8;8:85. doi: 10.1038/s41598-017-18391-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene clusters enriched in younger subjects. Locus maps display the genetic organization of clusters enriched in younger subjects. Reference strain name is indicated in parenthesis and genes are colored based on predicted function (uncolored genes represent hypothetical proteins). (a) Comparison of three types of sialidase clusters observed in our dataset with the corresponding reference strain indicated. (b) Locus map of the arabinofuranosidase cluster 1 detected in our dataset. Red outer boundary indicates the glycoside hydrolase predicted as extracellular. (c) Locus map of the exopolysaccharide biosynthesis cluster where homologues are located on the same contig in five out of 17 strains. (d) Plasmid pNAC3 homologue and (e) Region encoding a predicted Type VII secretion system.