Figure 5.

Ets-2 knockdown increases HIV-1-LTR expression. Jurkat cells were transfected with increasing amounts of the Ets-2 knockdown shRNA clones (0, 0.5, and 1 µg) and a constant amount (1 µg) of the reporter plasmids (A) pUC-BENN-CAT, (B) 2× RATS-CAT, or (C) 2× mutantRATS-CAT. The amount of transfected DNA per sample was retained to 2 µg by the addition of appropriate amounts of vector pLKO.1 DNA. The cells were cultured ±P/I as indicated. CAT assays were performed in cell extracts. The results are presented as the mean values (SD) from three independent experiments. Statistically significant differences are indicated by asterisks (*p < 0.05, one-way ANOVA). (D) Western blot analysis to verify the silencing of Ets-2 in the transfected cells. β-Tubulin protein levels were used as an internal control for equal loading. The results shown are representative of at least three independent experiments.