Fig. 4.

Biochemical validation of the length of RNA-binding channel within the Cg-mtEXO complex and Cg-Dss1 alone. a Schematic of the experiment with fluorescein-labeled RNA degradation by mtEXO in the presence of anti-fluorescein antibody that blocks RNA translocation. b, c Exoribonuclease activity assay of full-length Cg-mtEXO and Cg-Dss1, respectively, on the fluorescein-labeled substrate W30-F (see Supplementary Table 2 for sequence) in the presence and absence of the anti-fluorescein antibody. The reaction products at the time-points indicated on top of the gel were analyzed by 20% TBE-urea PAGE and scanned for fluorescent signal of the substrate