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. 2018 Jan 8;8:75. doi: 10.1038/s41598-017-18426-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of the usability of the reference points for landmark-based 3-D registration. (a–c) Landmark-based rigid registration of two tibial plateau samples in the Fiji software, using RP1-5 as landmarks. 3-D view of the (a) template and (b) model image after registration. (c) Merged view of the template and model images. Landmark-based affine registration of the same two samples in the 3D Slicer software, (d–g) using only RP1-5 as landmarks, and (h–k) using additional 2 landmarks (labelled with orange crosses) to RP1-5, placed on the medial end of the medial tibial plateau and the lateral end of the lateral tibial plateau on the 2-D section corresponding to RL3. Images show the transformed samples after registration. Example micro-CT images corresponding to RL3 of the samples set as the (d,h) fixed and (e,i) the moving volume. (f,j) Posterior and (g,k) top view of the two samples merged after registration. The 3-D volume shows the sample set as the moving volume, and the 2-D micro-CT section shows RL3 of the sample set as the fixed volume. Abbreviations: a: anterior, d: distal, l: lateral, m: medial, pr: proximal, po: posterior.