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. 2018 Jan 8;8:67. doi: 10.1038/s41598-017-18464-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Influence of rotavirus replicative ability on total MHCI expression in infected cells and bystander uninfected cells. Cells were mock-infected, infected with SA11-5S at a m.o.i. of 1, or exposed to a corresponding inoculum of inactivated SA11-5S (I-SA11-5S). After 16 h cells were fixed and permeabilized, stained for rotavirus (a) and total MHCI (b), and analysed by flow cytometry as described in the legend to Fig. 1. (c) The mean ± S.D. of the MHCI MFI from three biological replicates is shown.