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. 2018 Jan 8;8:5. doi: 10.1038/s41598-017-18501-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Screening of a panel of molecules. HUVECs were infected with P. aeruginosa wild-type strain in the presence of molecules targeting the bacteria (a,b) or targeting the eukaryotic cells (c,d) and their respective controls. To assess the effect of siRNA transfection in the cells, HUVECs were transfected two days before infection (e,f). A strain deficient for the production of the T3SS needle subunit (ΔpscF) was used as control. The kinetics of bright nuclei appearance (a,c,e) and bacteria growth (b,d,f) were simultaneously recorded by live-imaging and analysed. a.u. = arbitrary units.