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. 2018 Jan 8;8:5. doi: 10.1038/s41598-017-18501-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of cell damage kinetics with different bacteria. HUVECs were infected with 16 different bacteria and monitored by live-imaging. The percentages of bright nuclei were used to derive kinetics plots (a) and the corresponding Area Under the Curves (AUC) (b). Each point in the kinetics plots corresponds to the means of triplicates and AUC histograms represent the means of the AUCs obtained for the kinetics of each replicate. Error bars represent the standard deviation (n = 3). Stars indicate statistically significant differences between strains of the same species and NS a non-significant difference (one-way ANOVA, P < 0.05). Each of the kinetics plot replicates obtained with different bacterial species inducing “bright nuclei” were fitted with sigmoid model curves and the inflection point and curve steepness were calculated and represented as XY pairs (c). Means and standard deviations are represented.