FIGURE 1.

Establishment and characterization of TMZ-resistant GBM cell lines. (A,B) Cell viability analysis was performed to evaluate cytotoxicity of TMZ to T98G, T98G-R, U87, and U87-R cells under treatment with the indicated concentrations of TMZ for 72 h. (C,D) In vitro cell proliferation curves in response to TMZ treatment. (E) GBM cells were treated with or without TMZ for 48 h and cell cycle was examined by flow cytometry. (F,G) GBM cells subjected to wound healing assays. Cell migration rate was analyzed by ImageJ. (H) Expression of E-cadherin and Vimentin in GBM cells was examined by western blot. Above experiments were repeated three independent times with similar results. Each data point represents the mean ± SD. ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001.