FIGURE 3.

SCD1 modulates TMZ resistance in GBM cells in vitro. (A) The correlation between SCD1 mRNA expression and IC₅₀ values in six glioma cells was quantified by Spearman’s rank correlation. (B,C) qPCR and western blot confirmed increased SCD1 expression in T98G and U87 cells 48 h after transfection with SCD1 cDNA clone, versus transfection with the blank plasmid pcDNA3.1. (D,E) Cell viability assay was performed in T98G and U87 cells after overexpression of SCD1. (F,G) qPCR and western blot show si-SCD1 knockdown after 48 h transfection in T98G-R and U87-R cells. A non-specific siRNA was used in parallel.(H,I) T98G-R and U87-R cells transfected with si-SCD1 or si-control subjected to MTS assays. ^∗p < 0.05, ^∗∗p < 0.01. Experiments were performed three times with similar results.