FIGURE 5.

Effect of SCD1 on the Akt signaling pathway. (A) Western blot for the Akt signaling related proteins, Akt, GSK3β, and β-catenin in T98G, T98G-R, U87, and U87-R cells. (B) After T98G and U87 cells were transfected with pcDNA3.1 or pcDNA-SCD1 for 48 h, the total protein was extracted and analyzed by western blot with the indicated antibodies. (C) After T87G-R and U87-R cells were transfected with si-Ctrl or si-SCD1 for 48 h, the total protein was extracted and analyzed by western blot with the indicated antibodies. α-Tubulin was used as the internal control. All western blots are representative of results from at least three independent experiments.