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. 2018 Jan 8;8:11. doi: 10.1038/s41598-017-18324-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Immunofluorescence (IF) staining was used to examine the expression of the Dopamine transporter (Dat, red) in pABM-I@M treated MFs after transfection 7, 14 and 19 days. (b) The image analysis-based quantification of the number of Dat-expressing cells was normalized to the number of nuclei which were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, blue). (c) The quantification of dopamine analyzed by ELISA. Data are means ± standard deviation of three independent calculation of cell number ( $\geq$ 200). The asterisk (*) indicate statistical differences (p < 0.05) compared with control, and **indicate p < 0.001.