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. 2018 Jan 8;9:74. doi: 10.1038/s41467-017-02426-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Changes in cell surface pH in O. sinensis due to the activity of external carbonic anhydrase. a Acetazolamide (AZ), an inhibitor of external carbonic anhydrase (eCA), has a significant impact on cell surface pH. Light-dependent increases in cell surface pH were measured using a pH microelectrode. The addition of AZ largely inhibits the increase in cell surface pH. b Light-dependent increases in [O₂] for the cell shown in a. The addition of AZ substantially reduces the light-dependent increase in [O₂] around the cell. The inhibitory effect of AZ is rapidly reversed. c Mean changes in cell surface [H⁺] after illumination for 300 s following treatment with AZ. d Mean rate of O₂ evolution relative to control following treatment with AZ. Error bars represent s.e.m.