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. 2018 Jan 8;8:33. doi: 10.1038/s41598-017-18495-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reactivity of 14 different monoclonal CVB1-VP1 antibody clones against CVB1-3 viruses and CVB1 VP1 as well as mapping of their binding region in ELISA. (a) Screening of hybridoma supernatants against purified CVB1-3 viruses and CVB1 VP1 protein. Virus samples were heat-inactivated before coating. (b) Screening of hybridoma supernatants against CVB1 VP1, and the N- and C-terminal fragments of CVB3 VP1. Samples were run as duplicates, with 250 ng/well of antigen. The absorbance of blocked wells was subtracted from the sample absorbance values before plotting the data. Error bars show standard deviation of duplicates. In (b) N and C refers to terminal GST fusion constructs. Signal intensity differences between (a) and (b) are due to differing incubation times with substrate.