Table 1.
3A6 mainly recognizes EVs of the B species. A cell microarray including 20 different EVs and two non-EV viruses (Adenovirus C and Human Parechovirus 1) was immunostained with 3A6 in two independent laboratories to verify the recognition spectrum of the antibody. The results of chromogenic immunostaining by 3A6 are shown in comparison to those obtained with 5D8/121,24 are also shown. Recognition scale: +++ = strong, ++ = moderate, + = weak, − = negative.
| Virus | Cell line | Antibody | |||
|---|---|---|---|---|---|
| Species | Serotype | Strain | Rat anti-VP1 mAb | Mouse anti-VP1 mAb | |
| Clone 3A6 | Clone 5D8/1 | ||||
| EV B | CVB1 | ATCC | GMK | ++ | +++ |
| EV B | CVB2 | ATCC | GMK | ++ | +++ |
| EV B | CVB3 | ATCC | GMK | ++ | +++ |
| EV B | CVB4 | ATCC | GMK | ++ | +++ |
| EV B | CVB5 | ATCC | GMK | +++ | +++ |
| EV B | CVB6 | ATCC | GMK | ++ | ++ |
| EV B | Echo3 | PB-E3DiT23 | GMK | + | + |
| EV B | Echo4 | ATCC | GMK | ++ | + |
| EV B | Echo6 | ATCC | GMK | +++ | +++ |
| EV B | Echo9 | ATCC | GMK | ++ | ++ |
| EV B | Echo11 | ATCC | GMK | +++ | + |
| EV B | Echo30 | ATCC | A549 | +++ | +++ |
| EV B | CVA9 | ATCC | GMK | ++ | +++ |
| EV B | CVA9 | PB-CVA9V59 | RD | ++ | ++ |
| EV A | CVA2 | PB-CVA2V38 | RD | − | + |
| EV A | CVA4 | PB-CVA4V36 | RD | − | + |
| EV A | CVA5 | PB-CVA5V43 | RD | − | ++ |
| EV A | CVA6 | PB-CVA6V303V | RD | — | + |
| EV A | CVA10 | PB-CVA10V2530 | RD | — | + |
| EV A | CVA16 | PB-CVA16V55 | RD | — | + |
| EV A | CVA16 | ATCC | Vero | — | — |
| EV A | EV71 | PB-EV71Hus | GMK | — | — |
| EV C | PV3 | Sabin | GMK | ++ | ++ |
| Adeno | C | VR846 | HeLa | − | − |
| HPeV | HPeV1 | ATCC | A549 | − | − |