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. 2018 Jan 8;8:10. doi: 10.1038/s41598-017-18422-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The mid-IR spectra of different samples obtained from the G. lingzhi polysaccharide extraction process. (a) GL powder refers to the drying powder from wet G. lingzhi culture mycelia; (b) crude GLPS refers to the extracted crude polysaccharide sample from the dried powder in 70 °C of hot water; (c) GLPS after condensing refers to the condensing supernatant sample using rotary vacuum approach; (d) GLPS after ethanol precipitating refers to the condensed remnant sample after ethanol precipitating process; (e) GLPS after Sevag refers to the sample with proteins removed with the by Sevag method; and (f)GLPS after dialysis refers to the sample which removed small molecular impurity substances after dialyzing process.