Figure 2.

Osthole reduced APAP-induced oxidative stress. Mice were intraperitoneally treated with osthole (100 mg/kg) for 3 consecutive days. On the fourth day, APAP (300 mg/kg) and osthole were simultaneously administered to mice. Mice were sacrificed at 6 h post-APAP, and parameters of oxidative stress were determined. (A) Serum MDA; (B) Hepatic H₂O₂; (C) Hepatic GSH+GSSG; (D) Hepatic GSH; (E) GSSG-to-GSH ratio. (F) Relative mRNA levels of Nox2. Data are expressed as the mean±SEM. n=5–6 mice per group. ^*P<0.05, ^**P<0.01 compared to control. ^#P<0.05, ^##P<0.01 compared to APAP.