Figure 5.

The preventive effect of osthole against APAP-induced hepatotoxicity was independent of the cAMP/PKA pathway. (A, B) Mice were intraperitoneally treated with osthole (100 mg/kg) for 3 consecutive days. On the fourth day, APAP (300 mg/kg) and osthole were simultaneously administered to mice. Mice were sacrificed at indicated time points post-APAP. Hepatic cAMP and cGMP levels were determined. (C, D) H89 (10 mg/kg) was intraperitoneally administered to mice 1 h prior to APAP treatment on the fourth day. Serum ALT and AST levels at 6 h post-APAP were determined. (E) The mRNA levels of genes involved in inflammation and metabolism of APAP in the liver were quantified using real-time PCR at 6 h post-APAP. Data are expressed as the mean±SEM. n=4–6 mice per group. ^**P<0.01 compared to vehicle or control. ^#P<0.05, ^##P<0.01 compared to APAP.