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. 2017 Aug 3;39(1):97–106. doi: 10.1038/aps.2017.51

Figure 4.

Figure 4

Comparison of phosphorylation of Akt, eNOS and PI3K among the sham, IR, IR+V, IR+U, IR+U+W, IR+N+U and IR+W groups. (A) The Western blotting images of phosphorylation of Akt, eNOS and PI3K among 7 groups; (B) The gray-scale analysis phosphorylation of Akt, eNOS and PI3K among 7 groups. *P<0.05 vs the sham group. #P<0.05 vs the IR group. $P<0.05 vs the IR+U group. Akt, protein kinase B; eNOS, endothelial NO synthases; PI3K, phosphoinositide 3-kinase; IR, ischemic reperfusion; V, normal saline; N, KOR antagonist Nor-BNI; U, KOR agonist U50448H; W, PI3K inhibitors wortmannin; p-, phosphorylated.