Comparison of phosphorylation of Akt, eNOS and PI3K after HR of renal tubular epithelial cells among the blank, HR, HR+U, HR+U+W and HR + W groups. (A) The Western blotting images of phosphorylation of Akt, eNOS and PI3K after HR of renal tubular epithelial cells among five groups; (B) The gray-scale analysis of phosphorylation of Akt, eNOS and PI3K after HR of renal tubular epithelial cells among five groups. *P<0.05 vs the sham group. #P<0.05 vs the HR group. $P<0.05 vs the HR+U group. Akt, protein kinase B; eNOS, endothelial NO synthases; PI3K, Phosphoinositide 3-kinase; HR, hypoxia-reoxygenation; U, KOR agonist U50448H; W, PI3K inhibitors wortmannin; p-, phosphorylated.