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. 2017 Aug 3;39(1):35–47. doi: 10.1038/aps.2017.56

Figure 1.

Figure 1

Hypotonic challenge evoked WNK1 phosphorylation. (A) WNK1 (T58) phosphorylation (p-WNK1) was evoked by hypotonic solution exposure from 0.5 to 3 min. The hypotonic solution noticeably increased the WNK1 phosphorylation level within 0.5 min from 1.01±0.02 to 1.39±0.05 and up to 1.60±0.09 in 1 min (**P<0.01 vs isotonic group. Mean±SEM. n=6). A representative Western blot and densitometric analysis of p-WNK1 are shown. (B) Expression of WNK1 protein induced by the hypotonic solution. Densitometric analysis revealed that the hypotonic solution did not change WNK1 protein expression (P>0.05 vs isotonic group. Mean±SEM. n=5). (C and D) Compared with the isotonic solution, the hypertonic solution did not alter the p-WNK1 and WNK1 expression levels, whereas the hypotonic solution enhanced the p-WNK1 level (**P<0.01 vs Isotonic group. Mean±SEM. n=5). Abbreviations: Iso, isotonic solution; Hypo, hypotonic solution; hyper, hypertonic solution.