Figure 4.

WNK1 enhanced cell cycle transition induced by the hypotonic solution. (A) Hypotonic-induced cell cycle transition was detected by flow cytometric analysis. Densitometric analysis demonstrated that the hypotonic solution accelerated cell cycle transition from G₀/G₁ phase to S phase (^**P<0.01 vs vector in the isotonic group), which was further increased by overexpression of WNK1 (^##P<0.01 vs vector in the hypotonic group. Mean±SEM. n=7). (B) Silencing of WNK1 by WNK1-siRNA transfection reduced the cell cycle transition induced by the hypotonic solution. (^**P<0.01 vs negative in the isotonic group. ^##P<0.01 vs negative in the hypotonic group. Mean±SEM. n=7)