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. 2017 Aug 3;39(1):35–47. doi: 10.1038/aps.2017.56

Figure 6.

Figure 6

Effect of WNK1 on Akt phosphorylation. (A) Hypotonic challenge for 1 min significantly increased the Akt phosphorylation (p-Akt) level from 0.92±0.08 to 1.74±0.15 (**P<0.01 vs vector in the isotonic group), which was further enhanced by hWNK1 cDNA transfection for 48 h from 1.74±0.15 to 2.68±0.38 (##P<0.01 vs vector in the hypotonic group). The vector did not change the p-Akt level. A representative Western blot and densitometric analysis of p-WNK1 are shown. (B) Hypotonic solution exposure or overexpression of WNK1 did not alter Akt expression. A representative Western blot and densitometric analysis of Akt expression are shown. (C) Silencing of WNK1 significantly reduced p-Akt induced by the hypotonic solution from 1.63±0.17 to 1.19±0.13 (#P<0.05 vs negative in the hypotonic group). Negative siRNA did not change the p-Akt level. A representative Western blot and densitometric analysis of p-Akt are shown. (D) Silencing of WNK1 did not alter Akt expression. A representative Western blot and densitometric analysis of Akt expression are shown. Mean±SEM. n=6.