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. 2018 Jan 8;8:93. doi: 10.1038/s41598-017-18551-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of Sema4B in glioma cell lines. (A) Representative image of a western blot analysis of human glioma cell line extracts reveals expression of Sema4B in all cell lines tested. Actin was used as a loading control. The full-length blots are presented in Supplementary Fig. 3. (B) qPCR analysis of Sema4B mRNA in different glioma cell lines. Data are presented as mean ± s.e.m. (C) qPCR analysis of Sema4B mRNA under conditions of hypoxia. U87-MG and A127 were exposed to 1% or 21% oxygen for 6 h. Data are presented as mean ± s.e.m. The results in (A–C) are representative of three independent repetitions.