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. 2018 Jan 8;8:93. doi: 10.1038/s41598-017-18551-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CRISPR-cas9 mediated deletion of genomic locus of Sema4B results in reduced clonal potential. (A,B) Colony formation assay experiments (n = 3) were performed for cell line mutated with single CRISPR ((A), clone 1, 2 and CRISPR cont 1) or cells with CRISPR assisted deletion of the genomic locus of Sema4B ((B), del 1, del 2 or CRISPR control 2). 400 cells were seeded in each 6-well plate and stained with Giemsa after 18 days. Colonies were counted for each well; data represent the median of 9 plates in three experiments (the p values were calculated with the one-tailed Wilcoxon-Mann-Whitney Test * = 0.0328, *** = 0.0567668E-5).