Table 1.

Major protein components of the ctpA mutant from selected 2D-DIGE spots identified by mass spectrometry^a.

pI range/spot #b	Ratioc	Gene/protein	Geninfo identifier #d	Gene codee	# Peptides	Function
5–8/648	1.75	cysD	VBIRhiLeg32091_2444	RL1261	30	Sulfur assimilation
5–8/785, 792	2.06, 2.13	aroGf	VBIRhiLeg32091_3937	RL2686	33, 31	Phe, Tyr, and Trp biosynthesis
5–8/881	–1.21	aldA	VBIRhiLeg32091_3196	RL1966	17	Ala biosynthesis
5–8/1186	1.48	typA	VBIRhiLeg32091_5846	RL4506	51	Signal transduction
5–8/1203	–1.85	Periplasmic peptide-binding component	VBIRhiLeg32091_7097	pRL110243	52	Solute binding component of ABC transporter
5–8/1073	–1.82	Putative dipeptide/oligopeptide solute-binding component	VBIRhiLeg32091_5914	RL4575	33	Solute binding component of ABC transporter
5–8/640	1.64	Fructose-bisphosphate aldolase class I	VBIRhiLeg32091_5326	RL4012	32	Carbon metabolism
5–8/802	1.33	adhI	VBIRhiLeg32091_0524	pRL120524	32	Glutathione metabolism
3–6/314	2.18	Tricarboxylate transport protein TctABC	VBIRhiLeg32091_5210	RL3891	21	Transport of three carbon sugars
3–6/454	–2.81	Sugar ABC transporter, periplasmic sugar-binding protein	VBIRhiLeg32091_4922	RL3617	22	Transport of sugars
3–6/456, 465	–2.94, -2.82	Putative periplasmic substrate-binding ferrisiderophore receptorf	VBIRhiLeg32091_3966	RL2713	28, 27	Solute binding component of ABC transporter
3–6/267	–3.77	Amino acid ABC transporter, periplasmic amino acid-binding protein	VBIRhiLeg32091_4007	RL2753	27	Solute binding component of ABC transport system
3–6/299	1.62	ilvE	VBIRhiLeg32091_2512	RL1326	24	Ile, Leu, and Val biosynthesis
3–6/311	–3.14	Predicted erythritol ABC transporter 2, substrate-binding component	VBIRhiLeg32091_0201	pRL120200	13	Solute binding component of ABC transporter
3–6/262	1.95	Fumarylpyruvate hydrolase	VBIRhiLeg32091_4442	RL3169	14	Tyrosine metabolism
3–6/706	–1.57	groELf	VBIRhiLeg32091_2042	RL0883	46	Protein folding
3–6/485	–2.79	Leu, Ile, Val, Thr, and Ala binding protein	VBIRhiLeg32091_7251	pRL110400	24	Solute binding component of ABC transport system

^aSignificant difference (t-test) in spot intensity between wild type and ctpA mutant is for p ≤ 0.001. ^bProtein probability is 99.58%. ^cRatio of ctpA mutant vs. wild type average spot intensities for identified proteins separated by DIGE. ^dThe sequence accession number of identified proteins from Pathosystems Resource Integration Center (PATRIC). ^eGene code is according to the Sanger Centre annotation. ^fIdentified from two different DIGE spots.