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. 2018 Jan 8;8:102. doi: 10.1038/s41598-017-18260-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histological damage in the liver. (a) Representative photomicrographs of Hematoxylin Eosin (HE). Inflammatory foci (arrowheads), vacuolar degeneration, and necrosis are shown in the liver from eNOS ^−/−; sFlt1 mice. TUNEL (b) and immunohistochemistry against cleaved caspase 3 (c) in the liver. Scale bar indicates 100 µm. (d) The number of inflammatory foci is significantly increased in the liver from eNOS ^−/−; sFlt1 mice. (e) The score of ballooning hepatocytes. (f) Increased cleaved caspase 3 positive cells in the liver from eNOS ^−/−; sFlt1 mice. n = 5–8. Data are shown as box plot. ANOVA or Kruskal-Wallis test.