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. 2018 Jan 8;8:102. doi: 10.1038/s41598-017-18260-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inflammation in the liver. (a,b) Infiltrating neutrophils are visualized by Naphthol AS-D chloroacetate Esterase stain (blue). Scale bar indicates 100 µm. Number of neutrophil is increased by excessive sFlt1, which is further up-regulated by eNOS deletion. (c) The level of Mpo (myeloperoxidase) mRNA drastically increased in the liver from eNOS ^−/−; sFlt1 mice. (d) Expression of inflammation and pro-fibrotic related genes in the liver. N.S., not significant. n = 7–8. Data are shown as mean ± s.e.m or box plot. ANOVA or Kruskal-Wallis test.