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. 2018 Jan 8;8:102. doi: 10.1038/s41598-017-18260-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Markers of oxidative stress and hypoxia in the liver. (a) Representative photomicrographs of immunohistochemistry against 4-hydroxy-2-nonenal (4HNE). (b) Strong immunoreactive 4HNE is shown in the liver from eNOS ^−/−; sFlt1 mice. (c) Representative photomicrographs of immunohistochemistry against HO-1. (d) Strong immunoreactive HO-1 is shown in the liver from eNOS ^−/−; sFlt1 mice. (e,f) The levels of Hmox1 and Nqo1 mRNA in the liver. (g) Representative photomicrographs of immunohistochemistry against Hypoxia inducible factor 1α (HIF1α) in the liver. n = 5–8. Data are shown as mean ± s.e.m or box plot. ANOVA or Kruskal-Wallis test.