Fig. 4.

Expression of hTERT, miR-615-3p, HOXC5, PBX1–4, and MEIS1–3 in WA01 human ES cells upon neural induction. a A schematic representation of the human ES cells neural differentiation process in monolayer culture. The details are described under “Methods”. b The expression of pluripotency genes (NANOG and OCT-4) and neurodevelopmental gene PAX6 during neural differentiation (passage 0–4) in WA01 embryonic stem cells were quantified by real-time RT-PCR. The expression of hTERT mRNA. c miR-615-3p (d) and HOXC5 mRNA (e), during neural differentiation (passage 0–4) in WA01 cells was quantified by real-time RT-PCR as indicated. f The expression of PBX1–4 and MEIS1–3 in P0 and P3 of WA01 neural differentiation was quantified by real-time RT-PCR as indicated