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. 2018 Jan 8;8:62. doi: 10.1038/s41598-017-18390-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Formation and time evolution of the structure and size of prefibrillar aggregates as monitored at pH 8.7 under non-agitated conditions. Time-dependent changes in (a) CD spectra and (b) DLS size distributions. In panel a, the spectrum obtained in 10 mM NaOH is also shown by a dashed line as a reference. (c) Time course of molar ellipticity at 216 nm ([θ ₂₁₆]) and the position of the main peak in the size distribution of DLS. The black and red continuous lines show the fit curves obtained using equations 2 or 5, respectively. The inset shows a magnified view of the early time of the CD time-course plot.